Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US6482937

Title:Porcine Oct-4 promoter
Inventors:Baetscher, Manfred W.; Portland, OR, USA
Akiyoshi, Donna E.; Upton, MA, USA
Kaplan, Ruth A.; Tewsbury, MA, USA
Summary:This invention introduces novel porcine stem cells composed of a marker gene under an Oct-4 promoter, methods and uses thereof. Provided herein are processes by which pluripotent porcine embryonic stem cells may be isolated and propagated by use of a selectable marker gene which is inserted into the genetic material of the cells, and which permits the survival and growth of the porcine embryonic stem cells. Further disclosed are methods by which the selectable marker gene is inserted such that it may be regulated by a control or promoter polynucleotide sequence in the embryonic stem cells, such as by the porcine Oct-4 promoter sequence. Claims of the invention include a transgenic pig which may constitute a source of the pluripotent cells.
Abstract:The present invention provides for a method of isolating and/or propagating porcine stem cells, more specifically pluripotential porcine embryonic stem cells. The pluripotential cells are isolated and/or propagated by the use of a selectable marker gene which is inserted into the genetic material of the cells, and which permits the survival and growth of the porcine embryonic stem cells. The selectable marker gene is inserted so as to be regulated by a control or promoter polynucleotide sequence in the embryonic stem cells, for example the promoter polynucleotide sequence being the porcine Oct-4 promoter sequence of the present invention. The invention also provides for a transgenic pig which will constitute a source of the pluripotent cells.
US Patent Website:Click Here for Full Text of Patent
Title Number:US6482937
Application Number:US1997000948113
Date Filed:09/10/1997
Date Published:19/11/2002
Assignee:BioTransplant, Inc., Charlestown, MA, USA


 
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