Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US6485971

Title:Keratinocyte stem cells
Inventors:Kaur, Pritinder; East Melbourne, Australia
Simmons, Paul J.; East Melbourne, Australia
Summary:Described herein are methods of enriching a viable subpopulation of epidermal cells, namely, human keratinocyte stem cells (KSCs) or transit amplifying (TA) cells, to a high degree of purity, such that the subpopulation exhibits an altered proliferative potential when compared to an unfractionated population of epidermal cells. The invention relates to methods by which such enrichment may be successfully achieved on the basis of a cell surface component whose expression is proliferation-related in conjunction with an integrin such as the ?a6?4 integrin. Further disclosed are the means by which cells are harvested from an epithelium, and by which a transferrin receptor may be used as the cell surface component that is proliferation related. Therapeutic applications are included, such as in the formation of epidermal layers and in the starting of new cell populations for genetic modification in gene therapy.
Abstract:Enrichment for human Keratinocyte Stem Cells KSCs to a high degree of purity can be successfully achieved on the basis of a cell surface component whose expression is proliferation-related in conjunction with an integrin such as the .alpha..sub.6.beta..sub.4 integrin. Transferrin receptor may be used as the cell surface component that is proliferation related. Enrichment of Transit amplifying cells can also be achieved by use of a variation of this method. The enrichment follows on from harvesting of cells from an epithelium, preferably rich in stem cells.
US Patent Website:Click Here for Full Text of Patent
Title Number:US6485971
Application Number:US2000000664437
Date Filed:18/09/2000
Date Published:26/11/2002
Assignee:Peter MacCallum Cancer Institute, Victoria, Australia


 
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