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Stem Cell Related Patent Number US6497875

Title:Multilayer skin or dermal equivalent having a layer containing mesenchymal stem cells
Inventors:Sorrell, J. Michael; Cleveland Heights, OH, USA
Caplan, Arnold I.; Cleveland Heights, OH, USA
Summary:This invention introduces novel multilayer skin equivalents composed of a scaffold layer containing dermis-forming cells, particularly mesenchymal stem cells, and a keratinocyte layer. The dermis-forming cells and keratinocytes are described as being preferably autologous, and the dermis-forming cells are described as being human mesenchymal stem cells (MSCs), dermal fibroblasts such as papillary or reticular dermal fibroblasts, or mixtures thereof. The scaffold is described as being preferably type I collagen alone, or types I and II collagen in combination. Further disclosed are properties of a multilayer skin equivalent having a scaffold layer containing an extracellular matrix component comprising papillary dermal fibroblasts in laminar relationship with a layer of extracellular matrix component composed of reticular dermal fibroblasts, and a keratinocyte layer. Also provided are properties of a bioactive agent that promotes adhesion or angiogenesis, as well as a bioactive agent that enhances proliferation, commitment or differentiation of mesenchymal stem cells into dermal components, either in vitro or in vivo. Also included are properties of an injectable composition containing dermis-forming cells and an extracellular matrix component in a pharmaceutically acceptable injectable carrier.
Abstract:A multilayer skin equivalent is formed having a scaffold layer containing dermis-forming cells, and a keratinocyte layer. The dermis-forming cells and keratinocytes are preferably autologous, and the dermis-forming cells can be human mesenchymal stem cells (MSCs), dermal fibroblasts (e.g., papillary or reticular dermal fibroblasts) or mixtures thereof. The scaffold is preferably type I collagen alone, or types I and II collagen in combination. Also formed is a multilayer skin equivalent having a scaffold layer containing a layer of extracellular matrix component containing papillary dermal fibroblasts in laminar relationship with a layer of extracellular matrix component containing reticular dermal fibroblasts, and a keratinocyte layer. A multilayer dermal equivalent is provided having a layer of extracellular matrix component containing papillary dermis-forming cells and a layer of extracellular matrix component containing reticular dermis-forming cells. In another embodiment, the dermal equivalent has a layer containing MSCs and a layer selected from a layer of extracellular matrix component containing papillary dermis-forming cells and a layer of extracellular matrix component containing reticular dermis-forming cells, and optionally a keratinocyte layer. In the skin and dermal equivalents, at least one layer may contain an agent that promotes adhesion or angiogenesis. Also present may be a bioactive agent that enhances proliferation, commitment or differentiation of mesenchymal stem cells into dermal components, either in vitro or in vivo. An injectionable composition is also provided containing dermis-forming cells and an extracellular matrix component in a pharmaceutically acceptable injectable carrier.
US Patent Website:Click Here for Full Text of Patent
Title Number:US6497875
Application Number:US1998000171445
Date Filed:26/10/1998
Date Published:24/12/2002
Assignee:Case Western Reserve University, Cleveland, OH, USA


 
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