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Stem Cell Related Patent Number US6503712
| Title: | Methods and compositions for preparing a genomic library for knockout targeting vectors | | Inventors: | Thukral, Sushil K.; Thousand Oaks, CA, USA | | Summary: | This invention introduces novel methods and compositions for the preparation of a genomic library, specifically a mouse genomic library, for producing knock-out targeting vectors by arraying into pools bacteria transformed with the yeast-Escherichia coli shuttle vector comprising mouse genomic DNA fragments. The system is directed toward the production of gene targeting constructs by homologous recombination, and by methods generating transgenic animals. Further disclosed are the procedures for preparing a genomic library for use in producing knockout targeting vectors, including details of genomic DNA fragments, bacterial selection markers, yeast replication, and the amplification of shuttle vectors. | | Abstract: | The present invention is directed to methods for producing gene targeting constructs by homologous recombination using mouse genomic libraries arrayed in yeast shuttle vectors. The invention is also directed to methods of using targeting constructs made by the methods to generate transgenic animals. | | US Patent Website: | Click Here for Full Text of Patent | | Title Number: | US6503712 | | Application Number: | US2000000569975 | | Date Filed: | 10/05/2000 | | Date Published: | 07/01/2003 | | Assignee: | Amgen Inc., Thousand Oaks, CA, USA |
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