Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US6506574

Title:Hepatocyte lineage cells derived from pluripotent stem cells
Inventors:Rambhatla, Lakshmi; Redwood City, CA, USA
Carpenter, Melissa K.; Castro Valley, CA, USA
Summary:Described herein is a differentiated cell population which has phenotypic characteristics of liver cells and which is obtained by culturing pluripotent stem cells in the presence of a hepatocyte differentiation agent. Central to the invention is the discovery that when pluripotent stem cells are cultured in the presence of a hepatocyte differentiation agent, a population of cells is derived that has a remarkably high proportion of cells with phenotypic characteristics of liver cells. Described herein are methods by which human embryonic stem cells are allowed to form embryoid bodies which then combine with the differentiation agent n-butyrate, optionally supplemented with maturation factors. In another embodiment of the invention, n-butyrate is added to human embryonic stem cells in a feeder-free culture. Claims of the invention include a highly uniform cell population that is obtained and which is predominated by cells with morphological features of hepatocytes, expressing surface markers characteristic of hepatocytes, and having enzymatic and biosynthetic activity important for liver function. Therapeutic applications include an abundant source of cells of the hepatocyte lineage for use in drug screening and in the replenishing of liver function.
Abstract:It has been discovered that when pluripotent stem cells are cultured in the presence of a hepatocyte differentiation agent, a population of cells is derived that has a remarkably high proportion of cells with phenotypic characteristics of liver cells. In one example, human embryonic stem cells are allowed to form embryoid bodies, and then combined with the differentiation agent n-butyrate, optionally supplemented with maturation factors. In another example, n-butyrate is added to human embryonic stem cells in feeder-free culture. Either way, a remarkably uniform cell population is obtained, which is predominated by cells with morphological features of hepatocytes, expressing surface markers characteristic of hepatocytes, and having enzymatic and biosynthetic activity important for liver function. Since stem cells readily proliferate in culture, this system provides an abundant source of cells of the hepatocyte lineage for a variety of applications, such as drug screening, and replenishing liver function in the context of clinical treatment.
US Patent Website:Click Here for Full Text of Patent
Title Number:US6506574
Application Number:US2001000872182
Date Filed:31/05/2001
Date Published:14/01/2003
Assignee:Geron Corporation, Menlo Park, CA, USA


 
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