Methods comprising apoptosis inhibitors for the generation of transgenic pigs

Stem Cell Clinic
Patient Application
FAQ
Contact
Locations
News
Videos
Research
Patents
3D Culture/Scaffold
Administration
Differentiation
Expansion
Extraction/Preservation
Mobilization
Type
USE
 


Stem Cell Related Patent Number US6514752

Title:Homologous recombination for universal donor cells and chimeric mammalian hosts
Inventors:Kucherlapati, Raju; Darien, CT, USA
Koller, Beverly H.; Carrboro, NC, USA
Smithies, Oliver; Chapel Hill, NC, USA
Dubridge, Robert B.; Belmont, CA, USA
Greenburg, Gary; San Carlos, CA, USA
Capon, Daniel J.; Hillsborough, CA, USA
Williams, Steven R.; San Francisco, CA, USA
Arbones De Rafael, Mariona Lourdes; Barcelona, Spain
Summary:Described herein are genetically engineered mammalian cells lacking functional MHC antigens. The invention relates to the use of homologous recombination in the inactivation of genes, particularly genes associated with MHC antigens such as the beta2-microglobulin gene, which is inactivated for reducing or eliminating the expression of functional Class I MHC antigens. Claims of the invention include use of the resulting cells as universal donor cells. Further disclosed are methods by which embryonic stem cells may be modified by homologous recombination for use in producing chimeric or transgenic mammalian hosts, which may be used as source of universal donor organs, or as models for drug and transplantation therapies. Also provided are methods by which homologous recombination may be used in non-transformed mammalian somatic cells. Therapeutic applications are included, such as in transplantation and as universal donor cells.
Abstract:Homologous recombination is employed to inactivate genes, particularly genes associated with MHC antigens. Particularly, the .beta..sub.2 -microglobulin gene is inactivated for reducing or eliminating the expression of functional Class I MHC antigens. The resulting cells may be used as universal donor cells. In addition, embryonic stem cells may be modified by homologous recombination for use in producing chimeric or transgenic mammalian hosts, which may be used as source of universal donor organs, or as models for drug and transplantation therapies. Methods for homologous recombination in non-transformed mammalian somatic cells are also described.
US Patent Website:Click Here for Full Text of Patent
Title Number:US6514752
Application Number:US1995000443613
Date Filed:18/05/1995
Date Published:04/02/2003
Assignee:Cell Genesys, Inc., Foster City, CA, USA


 
Copyright © 2007 The Institute for Cellular Medicine  6/18/2021