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| Title: | Homologous recombination for universal donor cells and chimeric mammalian hosts |
| Inventors: | Kucherlapati, Raju; Darien, CT, USA Koller, Beverly H.; Carrboro, NC, USA Smithies, Oliver; Chapel Hill, NC, USA Dubridge, Robert B.; Belmont, CA, USA Greenburg, Gary; San Carlos, CA, USA Capon, Daniel J.; Hillsborough, CA, USA Williams, Steven R.; San Francisco, CA, USA Arbones De Rafael, Mariona Lourdes; Barcelona, Spain |
| Summary: | Described herein are genetically engineered mammalian cells lacking functional MHC antigens. The invention relates to the use of homologous recombination in the inactivation of genes, particularly genes associated with MHC antigens such as the beta2-microglobulin gene, which is inactivated for reducing or eliminating the expression of functional Class I MHC antigens. Claims of the invention include use of the resulting cells as universal donor cells. Further disclosed are methods by which embryonic stem cells may be modified by homologous recombination for use in producing chimeric or transgenic mammalian hosts, which may be used as source of universal donor organs, or as models for drug and transplantation therapies. Also provided are methods by which homologous recombination may be used in non-transformed mammalian somatic cells. Therapeutic applications are included, such as in transplantation and as universal donor cells. |
| Abstract: | Homologous recombination is employed to inactivate genes, particularly genes associated with MHC antigens. Particularly, the .beta..sub.2 -microglobulin gene is inactivated for reducing or eliminating the expression of functional Class I MHC antigens. The resulting cells may be used as universal donor cells. In addition, embryonic stem cells may be modified by homologous recombination for use in producing chimeric or transgenic mammalian hosts, which may be used as source of universal donor organs, or as models for drug and transplantation therapies. Methods for homologous recombination in non-transformed mammalian somatic cells are also described. |
| US Patent Website: | Click Here for Full Text of Patent |
| Title Number: | US6514752 |
| Application Number: | US1995000443613 |
| Date Filed: | 18/05/1995 |
| Date Published: | 04/02/2003 |
| Assignee: | Cell Genesys, Inc., Foster City, CA, USA |