Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US6537542

Title:Targeted introduction of DNA into primary or secondary cells and their use for gene therapy and protein production
Inventors:Treco, Douglas; Arlington, MA, USA
Heartlein, Michael W.; Boxborough, MA, USA
Selden, Richard F; Wellesley, MA, USA
Summary:This invention describes a method of gene or DNA targeting in cells of vertebrate, particularly mammalian, origin, such that DNA is introduced into the primary or secondary cells of vertebrate origin through homologous recombination or targeting of the DNA at preselected sties. Further disclosed are the precise methods of homologous recombination of the primary or secondary cells, as well as the methods of turning on a gene that is present in primary cells, secondary cells or immortalized cells of vertebrate origin, which is normally not expressed in the cells or is not expressed at significant levels in the cells. Specific DNA sequences are discussed, as are the therapeutic uses of such procedures.
Abstract:The present invention relates to a method of gene or DNA targeting in cells of vertebrate, particularly mammalian, origin. That is, it relates to a method of introducing DNA into primary or secondary cells of vertebrate origin through homologous recombination or targeting of the DNA, which is introduced into genomic DNA of the primary or secondary cells at a preselected site. The present invention further relates to primary or secondary cells, referred to as homologously recombinant (HR) primary or secondary cells, produced by the present method and to uses of the homologously recombinant primary or secondary cells. The present invention also relates to a method of turning on a gene present in primary cells, secondary cells or immortalized cells of vertebrate origin, which is normally not expressed in the cells or is not expressed at significant levels in the cells.
US Patent Website:Click Here for Full Text of Patent
Title Number:US6537542
Application Number:US2000000549697
Date Filed:14/04/2000
Date Published:25/03/2003
Assignee:Transkaryotic Therapies, Inc., Cambridge, MA, USA


 
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