|
|
|
Stem Cell Related Patent Number US6541247
| Title: | Method of isolating ependymal neural stem cells | | Inventors: | Frisen, Jonas; Stockholm, Sweden
Janson, Ann Marie; Stockholm, Sweden
Johansson, Clas; Stockholm, Sweden
Momma, Stefan; Sp.ang.nga, Sweden
Clarke, Diana; Stockholm, Sweden
Zhao, Ming; Solna, Sweden
Lendahl, Urban; Sundbyberg, Sweden
Delfani, Kioumars; Solna, Sweden | | Summary: | Described herein are methods of isolating ependymal neural CNS stem cells from a post-natal animal via the dissociation of CNS tissue from the animal for cells expressing the Notch1 receptor. Various assays and methods of screening, dissociation, cell recovery and labeling of the ependymal cells are disclosed. Applications are provided in the preparation of a medicament for the treatment of CNS disorders by such methods of isolation and screening. | | Abstract: | The present invention relates to a method of isolating ependymal neural CNS stem cells from a post-natal animal or a human, which method comprises the steps of
(a) screening single cells obtained by dissociating CNS tissue from said animal for cells exhibiting at least one characteristic of an ependymal neural stem cell; and
(b) recovering the cells that exhibit the characteristic or characteristics screened for in step (a).
The screening may be performed for a specific cell surface protein or by previously labeling the ependymal cells.
The invention also relates to isolated ependymal neural CNS stem cells, in vitro and in vivo assays based on the findings according to the invention and various uses of the ependymal neural stem cells according to the invention. | | US Patent Website: | Click Here for Full Text of Patent | | Title Number: | US6541247 | | Application Number: | US1998000104772 | | Date Filed: | 25/06/1998 | | Date Published: | 01/04/2003 | | Assignee: | Neuronova AB, Stockholm, Sweden |
|
|
