Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US6541249

Title:Immortalized human stromal cell lines
Inventors:Wager, Ruth E.; Rockville, MD, USA
Ourmanova, Maria; Boyds, MD, USA
Summary:Described herein are novel immortalized human stromal cell lines, methods and uses thereof. The invention relates to a method for the culture of hematopoietic cells and the production of human cytokines using immortalized human stromal cell lines such that the ability of the hematopoietic stem cells to self-replicate and differentiate, and of their progenitors to expand and differentiate, is maintained by the media. Further disclosed are irradiated and non-irradiated immortalized human stromal cells that synthesize cytokines, such as, IL-7, IL-8, IL-11, FLT3L, SCF, LIF, M-CSF, TGF-beta 1, GM-CSF, MIP-1alpha, G-CSF, and MCP-1. Applications in the culturing of such hematopoietic cells and for the production of human cytokines are included.
Abstract:A method for the culture of hematopoietic cells and/or production of human cytokines using immortalized human stromal cell lines is provided. These immortalized stromal cell lines condition media such that the ability of the hematopoietic stem cells to self-replicate and/or differentiate is maintained by the media or the ability of the committed progenitors to expand and/or differentiate is maintained by the media. Further provided are irradiated and non-irradiated immortalized human stromal cells that synthesize cytokines, such as, IL-7, IL-8, IL-11, FLT3L, SCF, LIF, M-CSF, TGF-beta 1, GM-CSF, MIP-1alpha, G-CSF, and MCP-1.
US Patent Website:Click Here for Full Text of Patent
Title Number:US6541249
Application Number:US2000000740789
Date Filed:21/12/2000
Date Published:01/04/2003
Assignee:Human Genome Sciences, Inc., Rockville, MD, USA


 
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