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| Title: | Retroviral gene therapy vectors and therapeutic methods based thereon |
| Inventors: | Riviere, Isabelle; Boston, MA, USA Cohen, Lawrence K.; Oakland, CA, USA Guild, Brad; Concord, MA, USA Rafield, Lori F.; San Francisco, CA, USA Robbins, Paul; Mt. Lebanon, PA, USA Mulligan, Richard C.; Lincoln, MA, USA |
| Summary: | This invention describes recombinant retroviral vectors useful for nonselectively transducing cells in gene therapy, containing 5' and 3' long terminal repeats derived from a retrovirus or from a murine leukaemia virus. Further disclosed are retroviral vectors which include an insertion site for genes of interest and are capable of expressing high levels of the protein derived from the genes of interest in a wide variety of transfected cell types. Also included are retroviral vectors lacking a selectable marker, thus rendering them suitable for human gene therapy in the treatment of a variety of disease states without the co-expression of a marker product, such as an antibiotic. Applications of these retroviral vectors are provided, specifically in the use of certain packaging cell lines. |
| Abstract: | Retroviral vectors are disclosed which include an insertion site for genes of interest and are capable of expressing high levels of the protein derived from the genes of interest in a wide variety of transfected cell types. Also disclosed are retroviral vectors lacking a selectable marker, thus rendering them suitable for human gene therapy in the treatment of a variety of disease states without the co-expression of a marker product, such as an antibiotic. These retroviral vectors are especially suited for use in certain packaging cell lines. |
| US Patent Website: | Click Here for Full Text of Patent |
| Title Number: | US6544771 |
| Application Number: | US1994000252710 |
| Date Filed: | 02/06/1994 |
| Date Published: | 08/04/2003 |
| Assignee: | Cell Genesys, Inc., Foster City, CA, USA Whitehead Institute for Biomedical Research, Cambridge, MA, USA |