Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US6548263

Title:Miniaturized cell array methods and apparatus for cell-based screening
Inventors:Kapur, Ravi; Gibsonia, PA, USA
Adams, Terri; Pittsburgh, PA, USA
Summary:Described herein are methods of producing a substrate for selective cell patterning and cell-based screening. The invention relates to a process by which reactive hydroxyl groups are contacted on a substrate surface with a hydroxyl-reactive bifunctional molecule, and cell repulsive or adhesive moieties are deposited via stencils on the substrate. The formation of a monolayer is described, as are methods of screening. Further disclosed are processes of selective differentiation of the stem cells screened according to this method in the creation of tissue-specific and organ-specific cell substrates in the therapeutic treatment of various diseases.
Abstract:The present invention describes novel methods for making a substrate for selective cell patterning, and the substrates themselves, wherein the method comprises contacting reactive hydroxyl groups on the surface of a substrate with a hydroxyl-reactive bifunctional molecule to form a monolayer, and using stencils to deposit cell repulsive or cell adhesive moieties in controlled locations on the cell culture substrate. Methods comprising selective differentiation of stem cells to create tissue specific and organ-specific cell substrates, as well as the cell substrates themselves are also provided.
US Patent Website:Click Here for Full Text of Patent
Title Number:US6548263
Application Number:US2000000540862
Date Filed:31/03/2000
Date Published:15/04/2003
Assignee:Cellomics, Inc., Pittsburgh, PA, USA


 
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