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Stem Cell Related Patent Number US6552246
| Title: | Transgenic mice comprising CD45 knockout | | Inventors: | Mak, Tak Wah; Toronto, Canada
Penninger, Josef Martin; Toronto, Canada | | Summary: | This invention introduces a nonhuman mammal and its progeny with suppressed expression of the gene encoding CD45. Specifically, the authors describe a mouse lacking expression of a particular CD45 isoform in certain cells of the immune system, such that the mouse may optionally contain a transgene encoding the CD45RO isoform. Further disclosed is a DNA sequence comprising at least exons 6-8 of a mouse CD45 DNA construct into which a marker gene sequence has been substituted for at least exon 6 and intron 6, such that this disruption prevents the expression of CD45 on B cells and T cells and results in decreased cytotoxic T cell response. Specific DNA sequences are delineated, as are applications in the screening of drugs for their immunomodulatory effects. | | Abstract: | A mammal lacking expression of particular CD45 isoform in certain cells of the immune system is provided. The mammal may optionally contain a transgene encoding the CD45RO isoform. Also provided are methods of using these mammals. | | US Patent Website: | Click Here for Full Text of Patent | | Title Number: | US6552246 | | Application Number: | US1995000451318 | | Date Filed: | 26/05/1995 | | Date Published: | 22/04/2003 | | Assignee: | University Health Network, Toronto, Canada |
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