Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US6552246

Title:Transgenic mice comprising CD45 knockout
Inventors:Mak, Tak Wah; Toronto, Canada
Penninger, Josef Martin; Toronto, Canada
Summary:This invention introduces a nonhuman mammal and its progeny with suppressed expression of the gene encoding CD45. Specifically, the authors describe a mouse lacking expression of a particular CD45 isoform in certain cells of the immune system, such that the mouse may optionally contain a transgene encoding the CD45RO isoform. Further disclosed is a DNA sequence comprising at least exons 6-8 of a mouse CD45 DNA construct into which a marker gene sequence has been substituted for at least exon 6 and intron 6, such that this disruption prevents the expression of CD45 on B cells and T cells and results in decreased cytotoxic T cell response. Specific DNA sequences are delineated, as are applications in the screening of drugs for their immunomodulatory effects.
Abstract:A mammal lacking expression of particular CD45 isoform in certain cells of the immune system is provided. The mammal may optionally contain a transgene encoding the CD45RO isoform. Also provided are methods of using these mammals.
US Patent Website:Click Here for Full Text of Patent
Title Number:US6552246
Application Number:US1995000451318
Date Filed:26/05/1995
Date Published:22/04/2003
Assignee:University Health Network, Toronto, Canada


 
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