Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US6555324

Title:Method to distinguish hematopoietic progenitor cells
Inventors:Olweus, Johanna; Fremont, CA, USA
Summary:This invention describes a novel method of identifying hematopoietic progenitor cell populations. The method relates to fluorescent labeling of the cells with markers for CD34 and CD38 as well as with markers for cell adhesion molecules and growth factor receptors, and then detecting the cells by flow cytometry. Central to the invention is the discovery that certain changes in the expression of various cell adhesion molecules and growth factor receptors on human progenitor cells (such as CD34+ /CD38+) were found to correlate with the progression of the cell from nonlineage-committed to lineage-committed. Further described are methods by which flow cytometry is employed in conjunction with one or more such cellular markers to identify and isolate each of the different progenitor cell populations.
Abstract:
US Patent Website:Click Here for Full Text of Patent
Title Number:US6555324
Application Number:US1993000147707
Date Filed:04/11/1993
Date Published:29/04/2003
Assignee:Becton Dickinson and Company, Franklin Lakes, NJ, USA


 
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