Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US6562619

Title:Differentiation of human embryonic germ cells
Inventors:Gearhart, John D.; Baltimore, MD, USA
Summary:Described herein is a method of producing human pluripotent embryonic germ cells. The invention relates to a method of culturing primordial germ cells and feeder cells in a medium containing factors that elevate cellular cAMP levels. Further disclosed are methods by which such primordial germ cells are isolated from human embryonic tissue, such as from the gonadal ridges of the human embryo, and cultured such that the resulting cells resemble embryonic stem cells or embryonic germ cells in morphology and pluripotency. Also provided are means by which the cells are then maintained several months in culture and can be genetically manipulated using transgenic technology to insert heterologous genetic material. Applications in the building of physiological biosensors are included.
Abstract:Primordial germ cells isolated from human embryonic tissue, such as from the gonadal ridges of human embryo, are disclosed. The primordial germ cells are cultured resulting in cells that resemble embryonic stem cells or embryonic germ cells in morphology and pluripotency. The cells are maintained several months in culture and can be genetically manipulated using transgenic technology to insert heterologous genetic material.
US Patent Website:Click Here for Full Text of Patent
Title Number:US6562619
Application Number:US2000000553640
Date Filed:20/04/2000
Date Published:13/05/2003
Assignee:The Johns Hopkins University School of Medicine, Baltimore, MD, USA


 
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