Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US6586251

Title:Methods of modifying eukaryotic cells
Inventors:Economides, Aris N.; Tarrytown, NY, USA
Murphy, Andrew J.; Croton-on-Hudson, NY, USA
Valenzuela, David M.; Yorktown Heights, NY, USA
Yancopoulos, George D.; Yorktown Heights, NY, USA
Summary:This invention describes methods of genetically modifying an endogenous gene or chromosomal locus in eukaryotic cells, for producing a genetically engineered non-human animal, for the creation of a large targeting vector. Disclosed is a method for engineering and utilizing large DNA vectors to target, via bacterial homologous recombination, and then to modify, endogenous genes and chromosomal loci in eukaryotic cells. These large DNA targeting vectors for eukaryotic cells (LTVECs) are derived from fragments of cloned genomic DNA larger than those typically used by other approaches intended to perform homologous targeting in eukaryotic cells. Further disclosed are the means for detecting eukaryotic cells in which the LTVEC has correctly targeted and modified the desired endogenous gene(s) or chromosomal locus (loci), specifically employing methods of quantitative assay to detect modification of the allele (MOA) in the eukaryotic cells for identification of those in which the endogenous gene or chromosomal locus has been genetically modified. Applications are also provided in the use of these cells to generate organisms bearing the genetic modification.
Abstract:A method for engineering and utilizing large DNA vectors to target, via homologous recombination, and modify, in any desirable fashion, endogenous genes and chromosomal loci in eukaryotic cells. These large DNA targeting vectors for eukaryotic cells, termed LTVECs, are derived from fragments of cloned genomic DNA larger than those typically used by other approaches intended to perform homologous targeting in eukaryotic cells. Also provided is a rapid and convenient method of detecting eukaryotic cells in which the LTVEC has correctly targeted and modified the desired endogenous gene(s) or chromosomal locus (loci) as well as the use of these cells to generate organisms bearing the genetic modification.
US Patent Website:Click Here for Full Text of Patent
Title Number:US6586251
Application Number:US2000000732234
Date Filed:07/12/2000
Date Published:01/07/2003
Assignee:Regeneron Pharmaceuticals, Inc., Tarrytown, NY, USA


 
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