Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US6593372

Title:Therapeutic uses for nitric oxide inhibitors
Inventors:Enikolopov, Grigori N.; Cold Spring Harbor, NY, USA
Peunova, Natalia I.; Cold Spring Harbor, NY, USA
Kuzin, Boris A.; Moscow, Russian Federation Cline, Hollis; Cold Spring Harbor, NY, USA
Michurina, Tatyana; Moscow, Russian Federation
Summary:Described herein are inhibitors of nitric oxide (NO) or nitric oxide synthase (NOS), and uses thereof. Central to the invention is the discovery that NO is an important growth regulator in an intact developing organism. The present invention relates to a method of increasing in a mammal a population of hematopoietic stem cells which are capable of undergoing normal hematopoiesis, differentiation and maturation in hematopoietic tissue, wherein the hematopoietic tissue is contacted with multiple doses of at least one inhibitor of NO, such as one or more inhibitors of NOS, thereby producing hematopoietic tissue having an increased population of hematopoietic stem cells which are capable of undergoing normal hematopoiesis, differentiation and maturation. Further disclosed are methods of increasing a population of cells in the S phase in a tissue of a mammal accordingly. Also provided are the means of regenerating tissue in an adult mammal via contacting a selected tissue, such as blood, skin, bone and digestive epithelium, or precursor cells of the selected tissue, with multiple doses of at least one inhibitor of NO, thereby inhibiting differentiation and inducing proliferation of cells of the tissue. Therapeutic applications in the regeneration of selected tissue in mammals are included.
Abstract:The present invention is based on the discovery that nitric oxide (NO) is an important growth regulator in an intact developing organism. In particular, the present invention relates to a method of increasing in a mammal a population of hematopoietic stem cells which are capable of undergoing normal hematopoiesis, differentiation and maturation in hematopoietic tissue, wherein the hematopoietic tissue is contacted with multiple doses of at least one inhibitor of NO, such as one or more inhibitors of nitric oxide synthase (NOS), thereby producing hematopoietic tissue having an increased population of hematopoietic stem cells which are capable of undergoing normal hematopoiesis, differentiation and maturation. The present invention also relates to a method of increasing a population of cells in S phase in a tissue of a mammal, comprising contacting the tissue with multiple doses of at least one inhibitor (one or more) of NO, such as an inhibitor of NOS. The invention also pertains to a method of regenerating tissue in an adult mammal comprising contacting a selected tissue (e.g., blood, skin, bone and digestive epithelium), or precursor cells of the selected tissue, with multiple doses of at least one inhibitor (one or more) of NO, thereby inhibiting differentiation and inducing proliferation of cells of the tissue.
US Patent Website:Click Here for Full Text of Patent
Title Number:US6593372
Application Number:US2001000999779
Date Filed:25/10/2001
Date Published:15/07/2003
Assignee:Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, USA


 
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