Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US6602711

Title:Method of making embryoid bodies from primate embryonic stem cells
Inventors:Thomson, James A; Madison, WI, USA
Marshall, Vivienne S.; Madison, WI, USA
Swiergiel, Jennifer J.; Roscoe, IL, USA
Summary:This invention describes a method for producing primate embryoid bodies from colonies of primate embryonic stem cells that are adhering to a substrate. The primate embryoid bodies are formed from the primate ES cells, which form clumps, which are then removed and incubated under conditions in which the ES cells coalesce into embryoid bodies. Precise means of development of embryoid bodies from the primate ES cells are further disclosed.
Abstract:Primate embryoid bodies are formed from primate ES cells. The ES cells form clumps. One then removes the clumps, as clumps, and permits incubation under non-adherent conditions. The development of embryoid bodies from primate ES cells is dependent on maintaining the aggregation of cells, as individualized cells will rapidly die.
US Patent Website:Click Here for Full Text of Patent
Title Number:US6602711
Application Number:US2000000510444
Date Filed:21/02/2000
Date Published:05/08/2003
Assignee:Wisconsin Alumni Research Foundation, Madison, WI, USA


 
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