Methods comprising apoptosis inhibitors for the generation of transgenic pigs |
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| Title: | Low oxygen culturing of central nervous system progenitor cells |
| Inventors: | Csete, Marie; Ann Arbor, MI, USA Doyle, John; South Pasadena, CA, USA Wold, Barbara J.; San Marino, CA, USA McKay, Ron; Bethesda, MD, USA Studer, Lorenz; New York, NY, USA |
| Summary: | This invention describes a method of increasing cell differentiation of undifferentiated mesencephalic precursor cells, and the precise conditions thereof. Conditions for promoting cell survival, proliferation, and/or cellular differentiation are provided. Such proliferation has been found to be promoted and apoptosis reduced when cells are grown in a lowered-oxygen environment as compared to environmental oxygen conditions that are traditionally employed in cell culture techniques. Further described are the means by which differentiation of precursor cells to specific fates was also enhanced in a lowered-oxygen environment wherein a much greater number and fraction of dopaminergic neurons were obtained when mesencephalic precursors were expanded and differentiated in lowered oxygen conditions. Thus at more physiological oxygen levels the proliferation and differentiation of CNS precursors is enhanced, and lowered oxygen is a useful adjunct for ex vivo generation of specific neuron types. Methods and compositions exploiting these findings are further disclosed. |
| Abstract: | The present invention relates to the growth of cells in culture under conditions that promote cell survival, proliferation, and/or cellular differentiation. The present inventors have found that proliferation was promoted and apoptosis reduced when cells were grown in lowered oxygen as compared to environmental oxygen conditions traditionally employed in cell culture techniques. Further, the inventors found that differentiation of precursor cells to specific fates also was enhanced in lowered oxygen where a much greater number and fraction of dopaminergic neurons were obtained when mesencephalic precursors were expanded and differentiated in lowered oxygen conditions. Thus at more physiological oxygen levels the proliferation and differentiation of CNS precursors is enhanced, and lowered oxygen is a useful adjunct for ex vivo generation of specific neuron types. Methods and compositions exploiting these findings are described. |
| US Patent Website: | Click Here for Full Text of Patent |
| Title Number: | US6610540 |
| Application Number: | US1999000425462 |
| Date Filed: | 22/10/1999 |
| Date Published: | 26/08/2003 |
| Assignee: | California Institute of Technology, Pasadena, CA, USA National Institutes of Health, Bethesda, MD, USA |