Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US6610540

Title:Low oxygen culturing of central nervous system progenitor cells
Inventors:Csete, Marie; Ann Arbor, MI, USA
Doyle, John; South Pasadena, CA, USA
Wold, Barbara J.; San Marino, CA, USA
McKay, Ron; Bethesda, MD, USA
Studer, Lorenz; New York, NY, USA
Summary:This invention describes a method of increasing cell differentiation of undifferentiated mesencephalic precursor cells, and the precise conditions thereof. Conditions for promoting cell survival, proliferation, and/or cellular differentiation are provided. Such proliferation has been found to be promoted and apoptosis reduced when cells are grown in a lowered-oxygen environment as compared to environmental oxygen conditions that are traditionally employed in cell culture techniques. Further described are the means by which differentiation of precursor cells to specific fates was also enhanced in a lowered-oxygen environment wherein a much greater number and fraction of dopaminergic neurons were obtained when mesencephalic precursors were expanded and differentiated in lowered oxygen conditions. Thus at more physiological oxygen levels the proliferation and differentiation of CNS precursors is enhanced, and lowered oxygen is a useful adjunct for ex vivo generation of specific neuron types. Methods and compositions exploiting these findings are further disclosed.
Abstract:The present invention relates to the growth of cells in culture under conditions that promote cell survival, proliferation, and/or cellular differentiation. The present inventors have found that proliferation was promoted and apoptosis reduced when cells were grown in lowered oxygen as compared to environmental oxygen conditions traditionally employed in cell culture techniques. Further, the inventors found that differentiation of precursor cells to specific fates also was enhanced in lowered oxygen where a much greater number and fraction of dopaminergic neurons were obtained when mesencephalic precursors were expanded and differentiated in lowered oxygen conditions. Thus at more physiological oxygen levels the proliferation and differentiation of CNS precursors is enhanced, and lowered oxygen is a useful adjunct for ex vivo generation of specific neuron types. Methods and compositions exploiting these findings are described.
US Patent Website:Click Here for Full Text of Patent
Title Number:US6610540
Application Number:US1999000425462
Date Filed:22/10/1999
Date Published:26/08/2003
Assignee:California Institute of Technology, Pasadena, CA, USA National Institutes of Health, Bethesda, MD, USA


 
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