Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US6617159

Title:Serum free medium for chondrocyte cells
Inventors:Cancedda, Ranieri; Genoa, Italy
Summary:Described herein is a serum-free medium for a cell culture, composed of growth factors, lipids, and fatty acids. Further disclosed are methods and uses of such a medium in the growth of chondrocyte and mesenchymal stem cells. Central to the invention is the concept of avoiding the risk of serum pathogens. The authors thus describe a serum-free medium composed of FGF-2, linoleic acid, ascorbic acid, B-mercaptoethanol, transferrin, dexamethasone, EGF, PDGFbb, insulin and albumin for the growth of chondrocytes, and a serum-free medium composed of FGF-2, LIF, SCF, pantotenate, biotin and selenium for the growth of mesenchymal stem cells. Also provided are methods of cell growth in such media. Therapeutic applications are included, such as in the development of products for inducing the formation of bone, cartilage and other connective tissue, particularly for wound healing and tissue repair.
Abstract:Serum free media for growth and proliferation of chondrocytes and mesenchymal stem cells in culture are provided. A serum free medium for growth of chondrocytes includes a serum free composition comprising FGF-2, linoleic acid, ascorbic acid, B-mercaptoethanol, transferrin and dexamethasone. Further the composition comprises EGF, PDGFbb, insulin and albumin. A method for growing chondrocytes in a serum free medium comprising the composition is also provided. Also provided for mesenchymal stem cell growth, is a serum free medium which includes a composition comprising FGF-2, LIF, SCF, pantotenate, biotin and selenium and method, therefore.
US Patent Website:Click Here for Full Text of Patent
Title Number:US6617159
Application Number:US2001000831161
Date Filed:11/06/2001
Date Published:09/09/2003
Assignee:Consorzio per la Gestione del Centro di Biotechnologie Avanzate, Genoa, Italy Istituto Nazionale per la Ricerca Sul Cancro, Genoa, Italy

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