Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US6645489

Title:Methods and devices for the long-term culture of hematopoietic progenitor cells
Inventors:Pykett, Mark J.; Boxford, MA, USA
Rosenzweig, Michael; Boston, MA, USA
Kaplan, Richard B.; Beverly Hills, CA, USA
Summary:This invention describes a process of long term, in vitro culture, maintenance, expansion and differenetiation of hematopoietic progenitor cells in the absence of exogenously added hematopoietic growth factors. Further disclosed are improved methods for the introduction of foreign genetic material into cells of hematopoietic origin, and to apparatus for performing these methods. Means are provided by which the hematopoietic progenitor cells are cultured on a three-dimensional porous biomaterial which is described as enhancing hematopoietic progenitor cell survival as well as an expansion of progenitor cell numbers and functionality, while maintaining progenitor cell pluripotency in the absence of exogenous growth factors. Claims of the invention include a high level of transduction supported by the three-dimensional porous biomaterial on cells cultured in such environment.
Abstract:The invention pertains to methods and devices for the long term, in vitroculture of hematopoietic progenitor cells in the absence of exogenously added hematopoietic growth factors, improved methods for the introduction of foreign genetic material into cells of hematopoietic origin, and to apparatus for performing these methods. The hematopoietic progenitor cells are cultured on a three-dimensional porous biomaterial. The three-dimensional porous biomaterial enhances hematopoietic progenitor cell survival and leads to an expansion of progenitor cell numbers and/or functionality, while maintaining progenitor cell pluripotency in the absence of exogenous growth factors. In addition, the three-dimensional porous biomaterial supports high level transduction on cells cultured upon such environment.
US Patent Website:Click Here for Full Text of Patent
Title Number:US6645489
Application Number:US2002000143540
Date Filed:10/05/2002
Date Published:11/11/2003
Assignee:Cytomatrix, LLC, Woburn, MA, USA


 
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