Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US6667034

Title:METHODS FOR REGULATING THE SPECIFIC LINEAGES OF CELLS PRODUCED IN A HUMAN HEMATOPOIETIC CELL CULTURE, METHODS FOR ASSAYING THE EFFECT OF SUBSTANCES ON LINEAGE-SPECIFIC CELL PRODUCTION, AND CELL COMPOSITIONS PRODUCED BY THESE CULTURES
Inventors:Palsson, Bernhard O.; La Jolla, CA, USA
Armstrong, R. Douglas; Ann Arbor, MI, USA
Clarke, Michael F.; Ann Arbor, MI, USA
Emerson, Stephen G.; Ann Arbor, MI, USA
Summary:This invention introduces a method of culturing human stem and progenitor cells to control lineage development. The invention relates to a method of bone marrow transplantation via obtaining a bone marrow tissue or blood sample from a donor, culturing the bone marrow tissue or blood sample, and implanting it in a donee. The bone marrow tissue or blood sample is described as comprising a mass of human stromal cells in a liquid culture medium which is replaced, either continuously or periodically, at a rate of approximately 1 ml of medium per ml of culture per 24 to 48 hour period, as metabolic products are removed and depleted nutrients are replenished while maintaining the culture under physiologically acceptable conditions. Therapeutic applications are included, such as in the treatment of AIDS and related T-cell diseases.
Abstract:Methods, including culture media conditions, which provide for in vitro human stem cell division and/or the optimization of human hematopoietic progenitor cell cultures and/or increasing the metabolism or GM-CSF secretion or IL-6 secretion of human stromal cells and/or a method for assaying the effect of a substance or condition on a human hematopoietic cell population, and/or depleting the malignant cell or T-cell and B-cell content of a human hematopoietic cell population are disclosed. The methods rely on culturing human stem cells and/or human hematopoietic progenitor cells and/or human stromal cells in a liquid culture medium which is replaced, preferably perfused, either continuously or periodically, at a rate of 1 ml of medium per ml of culture per about 24 to about 48 hour period, and removing metabolic products and replenishing depleted nutrients while maintaining the culture under physiologically acceptable conditions. Optionally, growth factors are added to the culture medium. The disclosed culture conditions afford improved methods for bone marrow transplantation.
US Patent Website:Click Here for Full Text of Patent
Title Number:US6667034
Application Number:US1997000857594
Date Filed:16/05/1997
Date Published:23/12/2003
Assignee:The Regents of the University of Michigan, Ann Arbor, MI, USA


 
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