Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US6670177

Title:Methods for enhanced virus-mediated DNA transfer using molecules with virus-and cell-binding domains
Inventors:Williams, David A.; Indianapolis, IN, USA
Summary:Described herein are methods for enhanced virus-mediated DNA transfer, by use of fibronectin (FN) or FN fragments with virus- and cell-binding domains. The invention relates to a process for increasomg the efficiency of transduction of hematopoietic and other cell populations by retroviruses involving infecting the cells in the presence of fibronectin or fibronectin fragments, which are described as significantly enhancing retroviral-mediated gene transfer into the cells, particularly hematopoietic cells including committed progenitors and primitive hematopoietic stem cells. Further disclosed are improved therapeutic methods for increasing transduction efficiency in somatic gene therapy.
Abstract:A method to increase the efficiency of transduction of hematopoietic and other cells by retroviruses includes infecting the cells in the presence of fibronectin or fibronectin fragments. The fibronectin and fibronectin fragments significantly enhance retroviral-mediated gene transfer into the cells, particularly hematopoietic cells including committed progenitors and primitive hematopoietic stem cells. The invention also provides improved methods for somatic gene therapy capitalizing on enhanced gene transfer, hematopoietic cellular populations, and novel constructs for enhancing retroviral-mediated DNA transfer into cells and their use.
US Patent Website:Click Here for Full Text of Patent
Title Number:US6670177
Application Number:US1998000043981
Date Filed:19/06/1998
Date Published:30/12/2003
Assignee:Advanced Research and Technology Institute, Indianapolis, IN, USA


 
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