Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US6709864

Title:Adipogenic differentiation of human mesenchymal stem cells
Inventors:Pittenger, Mark F.; Severna Park, MD, USA
Beck, Stephen C.; Reistertown, MD, USA
Summary:Described herein is a composition for producing adipocytes, comprised of isolated human mesenchymal stem cells in a biocompatible matrix. The invention relates to a composition which induces cells from the mesenchymal stem cell population to differentiate into the adipogenic lineage, and a process for inducing such differentiation. Further disclosed are constituents of the composition, including a glucocorticoid, a compound which stimulates cAMP production or inhibits cAMP degradation such as a phosphodiesterase inhibitor, and a compound which upregulates peroxisome proliferator activated receptor gamma (PPAR gamma) expression and increases its binding affinity to its DNA binding site. A process for isolating the adipocytes from remaining hMSCs is provided, as are therapeutic applications in the production of adipocytes for implantation into a patient for tissue augmentation following trauma or cosmetic surgery.
Abstract:A composition which comprises human mesenchymal stem cells which have the potential to differentiate into cells of more than one connective tissue type and a composition which induces cells from the mesenchymal stem cell population to differentiate into the adipogenic lineage, and a process for inducing such differentiation. The composition for inducing such differentiation comprises a glucocorticoid, a compound which stimulates cAMP production or inhibits cAMP degradation (such as a phosphodiesterase inhibitor), and/or a compound which upregulates peroxisome proliferator activated receptor .gamma. (PPAR .gamma.) expression and/or increases its binding affinity to its DNA binding site. The process can further include isolating the adipocytes from remaining hMSCs.
US Patent Website:Click Here for Full Text of Patent
Title Number:US6709864
Application Number:US2000000537003
Date Filed:28/03/2000
Date Published:23/03/2004
Assignee:Osiris Therapeutics, Inc., Baltimore, MD, USA


 
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