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Stem Cell Related Patent Number US6759039

Title:Culturing pancreatic stem cells having a specified, intermediate stage of development
Inventors:Tsang, Wen-Ghih; Sherman Oaks, CA, USA
Zheng, Tianli; Culver City, CA, USA
Huang, Chang Jiang; Los Angeles, CA, USA
Summary:This invention describes methods of preparing cell cultures of propagating pancreatic cells that retain the potential to produce pancreatic hormones. Central to the invention is the discovery that an intermediate, differentiated stage of pancreatic stem cells exist that can be propagated in a stable manner in successive serial passaging while maintaining insulin production in response to glucose. Such cells are described herein as advantageous in that they are both expandable and stable in culture and can be driven to late stage development such as prototype islet cells. Further disclosed are culturing techniques that select for these intermediate differentiated stage cells and selectively eliminate early or late stage pancreatic cells. Claims of the invention include a method of producing an aggregate of cultured pancreatic cells composed of an encapsulating mantle of ck-19 positive cells and an inner cell mass, wherein the aggregate comprises 50-5000 pancreatic cells and has a diameter of between 50 and 300 microns. Therapeutic applications emphasize the restoration of pancreatic endocrine function to a mammal.
Abstract:This invention relates to the discovery that an intermediate, differentiated stage of pancreatic stem cells exist that can be propagated in a stable manner in successive serial passaging while maintaining insulin production in response to glucose. These cells are advantageous in that they are both expandable and stable in culture and can driven to late stage development, i.e. prototype islet cells. This invention further provides for culturing techniques that select for these intermediate differentiated stage cells and selectively eliminates early or late stage pancreatic cells.
US Patent Website:Click Here for Full Text of Patent
Title Number:US6759039
Application Number:US2001000895585
Date Filed:29/06/2001
Date Published:06/07/2004
Assignee:AmCyte, Inc., Santa Monica, CA, USA


 
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