|
|
|
Stem Cell Related Patent Number US6783977
| Title: | Internal ribosome entry site and vector containing same | | Inventors: | Lopez Lastra, Marcelo; Lyons, France
Gabus-Darlix, Caroline; Chaponost, France
Darlix, Jean-Luc; Chaponost, France | | Summary: | This invention introduces expression vectors containing IRES and/or an encapsidation enhancer derived from a type C retrovirus other than the Friend Murine Leukemia Virus (FMLV) or the Moloney Murine Leukemia Virus (MoMLV). Central to the invention is a nucleotide sequence derived from all or part of the genomic RNA 5' end of a type C retrovirus except for FMLV and MoMLV as an internal ribosome entry site or as an element enabling or improving retrovirus vector packaging. Further disclosed is a vector comprising the nucleotide sequence, a viral particle generated from this vector, a cell comprising this vector or infected by the viral particle, their therapeutic use and a pharmaceutical composition containing them. Methods of transfection are included. | | Abstract: | The invention concerns the use of a nucleotide sequence derived from all or part of the genomic RNA 5' end of a type C retrovirus except for Friend murine leukaemia virus (FMLV) and Moloney murine leukaemia virus (MoMLV) as internal ribosome entry site or as element enabling or improving retrovirus vector packaging. The invention also concerns a vector comprising said nucleotide sequence, a viral particle generated from this vector, a cell comprising this vector or infected by the viral particle, their therapeutic use and a pharmaceutical composition containing them. The invention further concerns the use of a vector, a viral particle or a pharmaceutical composition for transfecting or infecting pluripotent stem cells. | | US Patent Website: | Click Here for Full Text of Patent | | Title Number: | US6783977 | | Application Number: | US1999000214124 | | Date Filed: | 17/03/1999 | | Date Published: | 31/08/2004 | | Assignee: | Institut National de la Sante et de la Recherche Medicale (INSERM), Paris, France |
|
|
