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| Title: | Methods and materials for the growth of primate-derived primordial stem cells in feeder-free culture |
| Inventors: | Bodnar, Andrea G.; Oshawa, Canada Chiu, Choy-Pik; Cupertino, CA, USA Gold, Joseph D.; San Francisco, CA, USA Inokuma, Margaret; San Jose, CA, USA Murai, James T.; San Bruno, CA, USA West, Michael D.; Boston, MA, USA |
| Summary: | This invention describes a cellular composition comprising undifferentiated primate primordial stem (pPS) cells proliferating on an extracellular matrix, wherein the composition is free of feeder cells. Methods and materials are provided for culturing primate-derived primordial stem cells, such that the cell culture medium for growing primate-derived primordial stem cells in a substantially undifferentiated state includes a low osmotic pressure and a low endotoxin basic medium that is effective to support the growth of primate-derived primordial stem cells. Further disclosed are the means by which the basic medium is combined with a nutrient serum effective to support the growth of primate-derived primordial stem cells and a substrate selected from the group consisting of feeder cells and an extracellular matrix component derived from feeder cells. The medium is further described as including non-essential amino acids, an anti-oxidant, and a first growth factor selected from the group consisting of nucleosides and a pyruvate salt. |
| Abstract: | Methods and materials for culturing primate-derived primordial stem cells are described. In one embodiment, a cell culture medium for growing primate-derived primordial stem cells in a substantially undifferentiated state is provided which includes a low osmotic pressure, low endotoxin basic medium that is effective to support the growth of primate-derived primordial stem cells. The basic medium is combined with a nutrient serum effective to support the growth of primate-derived primordial stem cells and a substrate selected from the group consisting of feeder cells and an extracellular matrix component derived from feeder cells. The medium further includes non-essential amino acids, an anti-oxidant, and a first growth factor selected from the group consisting of nucleosides and a pyruvate salt. |
| US Patent Website: | Click Here for Full Text of Patent |
| Title Number: | US6800480 |
| Application Number: | US2000000530346 |
| Date Filed: | 29/08/2000 |
| Date Published: | 05/10/2004 |
| Assignee: | Geron Corporation, Menlo Park, CA, USA |