Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US6800480

Title:Methods and materials for the growth of primate-derived primordial stem cells in feeder-free culture
Inventors:Bodnar, Andrea G.; Oshawa, Canada
Chiu, Choy-Pik; Cupertino, CA, USA
Gold, Joseph D.; San Francisco, CA, USA
Inokuma, Margaret; San Jose, CA, USA
Murai, James T.; San Bruno, CA, USA
West, Michael D.; Boston, MA, USA
Summary:This invention describes a cellular composition comprising undifferentiated primate primordial stem (pPS) cells proliferating on an extracellular matrix, wherein the composition is free of feeder cells. Methods and materials are provided for culturing primate-derived primordial stem cells, such that the cell culture medium for growing primate-derived primordial stem cells in a substantially undifferentiated state includes a low osmotic pressure and a low endotoxin basic medium that is effective to support the growth of primate-derived primordial stem cells. Further disclosed are the means by which the basic medium is combined with a nutrient serum effective to support the growth of primate-derived primordial stem cells and a substrate selected from the group consisting of feeder cells and an extracellular matrix component derived from feeder cells. The medium is further described as including non-essential amino acids, an anti-oxidant, and a first growth factor selected from the group consisting of nucleosides and a pyruvate salt.
Abstract:Methods and materials for culturing primate-derived primordial stem cells are described. In one embodiment, a cell culture medium for growing primate-derived primordial stem cells in a substantially undifferentiated state is provided which includes a low osmotic pressure, low endotoxin basic medium that is effective to support the growth of primate-derived primordial stem cells. The basic medium is combined with a nutrient serum effective to support the growth of primate-derived primordial stem cells and a substrate selected from the group consisting of feeder cells and an extracellular matrix component derived from feeder cells. The medium further includes non-essential amino acids, an anti-oxidant, and a first growth factor selected from the group consisting of nucleosides and a pyruvate salt.
US Patent Website:Click Here for Full Text of Patent
Title Number:US6800480
Application Number:US2000000530346
Date Filed:29/08/2000
Date Published:05/10/2004
Assignee:Geron Corporation, Menlo Park, CA, USA


 
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