Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US6809117

Title:Therapeutic uses of nitric oxide inhibitors
Inventors:Enikolopov, Grigori N.; Cold Spring Harbor, NY, USA
Summary:This invention describes the use of inhibitors of nitric oxide or nitric oxide synthase to increase populations of cells in the S phase in tissues. Central to the invention is the discovery that nitric oxide (NO) is an important growth regulator in an intact developing organism, and described herein are methods of increasing in a mammal a population of hematopoietic stem cells in bone marrow which are capable of undergoing normal hematopoiesis and differentiation. Further disclosed are the means by which bone marrow is contacted with an inhibitor of NO, such as an inhibitor of nitric oxide synthase (NOS), thereby producing bone marrow with an increased population of hematopoietic stem cells which are capable of undergoing normal hematopoiesis and differentiation. Therapeutic applications in the regeneration of selected mammalian tissue are included. This invention represents a continuation of additional applications filed by the same assignee and inventors.
Abstract:The present invention is based on the discovery that nitric oxide (NO) is an important growth regulator in an intact developing organism. In particular, the present invention relates to a method of increasing in a mammal a population of hematopoietic stem cells in bone marrow which are capable of undergoing normal hematopoiesis and differentiation, wherein the bone marrow is contacted with an inhibitor of NO, such as an inhibitor of nitric oxide synthase (NOS), thereby producing bone marrow having an increased population of hematopoietic stem cells which are capable of undergoing normal hematopoiesis and differentiation. The present invention also relates to a method of increasing a population of cells in S phase in a tissue of a mammal, comprising contacting the tissue with an inhibitor of NO, such as an inhibitor of NOS. The invention also pertains to a method of regenerating tissue in an adult mammal comprising contacting a selected tissue (e.g., blood, skin, bone and digestive epithelium), or precursor cells of the selected tissue, with an inhibitor of NO, thereby inhibiting differentiation and inducing proliferation of cells of the tissue.
US Patent Website:Click Here for Full Text of Patent
Title Number:US6809117
Application Number:US2002000288639
Date Filed:04/11/2002
Date Published:26/10/2004
Assignee:Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, USA


 
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