Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US6815185

Title:Methods of creating constructs useful for introducing sequences into embryonic stem cells
Inventors:Klein, Robert D.; Palo Alto, CA, USA
Brennan, Thomas J.; South San Francisco, CA, USA
Summary:Described herein are novel nucleotide constructs comprised of a sequence encoding a positive selection marker flanked by restriction enzyme sites, resulting in the creation of a single-stranded region. Further disclosed are DNA constructs useful for introducing sequences into and disrupting the function of a gene in a cell, particularly in an embryonic stem cell.
Abstract:This invention provides novel nucleotide constructs. Also provided are methods for making DNA constructs useful for introducing sequences into and disrupting the function of a gene in a cell, particularly an embryonic stem cell.
US Patent Website:Click Here for Full Text of Patent
Title Number:US6815185
Application Number:US2001000885816
Date Filed:19/06/2001
Date Published:09/11/2004
Assignee:None


 
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