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Stem Cell Related Patent Number US6821790

Title:Methods and apparatus for separation of biological fluids
Inventors:Mahant, Vijay; Murrieta, CA, USA
Doneen, Byron; Carlsbad, CA, USA
Summary:Described herein are methods of separating a cell-containing sample into a cell-depleted portion and a cell-containing portion comprised of at least one stem cell, a lymphocyte, and a leukocyte. The invention relates to a process by which the sample is received in a vessel with at least one flexible wall to which an additive and particles are added, wherein the additive substantially binds to at least one of the stem cell, lymphocyte, or leukocyte. Further disclosed are means by which the particles substantially bind to at least one of the stem cell, lymphocyte, or leukocyte and the additive, thereby producing a cell-containing network, after which the network is separated from the substantially cell-depleted portion by application of a magnetic force. Therapeutic applications are included, such as in clinical diagnostics.
Abstract:A method of separating a cell-containing sample into a substantially cell-depleted portion, and a cell-containing portion comprising at least one of a stem cell, a lymphocyte, and a leukocyte comprises a step in which the sample is received in a vessel with at least one flexible wall. In another step, an additive and particles are added to the sample, wherein the additive substantially binds to the at least one of the stem cell, lymphocyte, and leukocyte, and the particles and wherein the particles substantially bind to the at least one of the stem cell, lymphocyte, and leukocyte, and the additive, thereby producing a cell-containing network. In a further step, the network is separated from the substantially cell-depleted portion by applying a magnetic force.
US Patent Website:Click Here for Full Text of Patent
Title Number:US6821790
Application Number:US2001000949314
Date Filed:07/09/2001
Date Published:23/11/2004
Assignee:None


 
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