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Stem Cell Related Patent Number US6835867
| Title: | Allelic series of genomic modifications in cells | | Inventors: | Woychik, Richard P.; Orinda, CA 94563
Magnuson, Terry R.; Chapel Hill, NC 27514
Avner, Ellis D.; Beachwood, OH 44122
Thomas, James W.; Rockville, MD 20851 | | Summary: | This is a method of producing an allelic series of modifications in a gene from an in vitro culture comprised of isolated mouse embryonic stem cells which contain the particular gene of interest. Methods of isolation of the cells and modification of the gene are disclosed, by which successive genetic modifications, yielding to the series of modifications, are produced. | | Abstract: | The present invention relates to methods of producing an allelic series of modifications in genes of interest in a cell. In particular, the invention provides methods for using nucleic acid sequence-modifying agents (e.g., chemicals, electromagnetic radiation, etc.) to introduce modifications in any nucleic acid sequence in the genome of a cell. Also provided are sets of cells which contain at least one modification in any gene of interest. The methods and compositions of the invention are useful in determining the function of the gene of interest. | | US Patent Website: | Click Here for Full Text of Patent | | Title Number: | US6835867 | | Application Number: | US1998000103846 | | Date Filed: | 24/06/1998 | | Date Published: | 28/12/2004 | | Assignee: | None |
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