Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US6849594

Title:Purification and use of human recombinant cartilage oligomeric matrix protein
Inventors:Chen, Hui; Boston, MA, USA
02134 Lawler, John W.; Swampscott, MA, USA
Summary:Herein is described a new enzyme-linked immunosorbent assay kit comprised of purified human cartilage oligomeric matrix protein (hCOMP), including hCOMP which is prepared by purifying hCOMP in the presence of calcium. Further disclosed are methods and uses, such as the means by which hCOMP is purified in the presence of calcium, associated antibodies involved in the purification of hCOMP, ELISA kits comprising purified hCOMP, as well as compositions and implants comprising COMP. Also provided are methods of repairing or producing cartilage via the implanting of a composition containing COMP and a differentiation agent, as well as methods for making an implant for cartilage repair via the binding of a differentiation agent to hCOMP. Methods of transplanting chondrocytes and mesenchymal stem cells are provided, in which such cells are cultured in the presence of hCOMP. Therapeutic methods are included, such as in the detection and quantification of COMP and COMP antibodies.
Abstract:The invention relates to purified cartilage oligomeric matrix protein (COMP), such as human COMP (hCOMP), including hCOMP prepared by purifying hCOMP in the presence of calcium (e.g. under calcium replete conditions); methods of purifying COMP in the presence of calcium; antibodies to purified hCOMP; ELISA kits comprising purified hCOMP; compositions (e.g. implants) comprising COMP; methods of repairing or producing cartilage comprising implanting a composition comprising COMP and a differentiation agent; methods for making an implant for cartilage repair comprising binding a differentiation agent to hCOMP; methods of transplanting chondrocytes and mesenchymal stem cells comprising culturing the cells in the presence of hCOMP; methods of transplanting chondrocytes comprising culturing them in the presence of hCOMP; methods of mediating attachment of cells using differentiation agent-bound COMP; methods of preparing a cartilage repair composition comprising culturing and purifying COMP in the presence of calcium and adding it to a matrix; and assays and methods of detection and quantification of COMP (e.g. degraded COMP and non-degraded COMP) and anti-COMP antibodies in a sample.
US Patent Website:Click Here for Full Text of Patent
Title Number:US6849594
Application Number:US2000000606763
Date Filed:29/06/2000
Date Published:01/02/2005
Assignee:Lawler; John, New York, NY, USA Chen; Hui, New York, NY, USA


 
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