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Stem Cell Related Patent Number US6852532
| Title: | Method of isolating human neuroepithelial precursor cells from human fetal tissue | | Inventors: | Mayer-Proschel, Margot; Pittsford, NY, USA
Rao, Mahendra S.; Salt Lake City, UT, USA
Tresco, Patrick A.; Sandy, UT, USA
Messina, Darin J.; Salt Lake City, UT, USA | | Summary: | This is a novel system for isolating human neuroepithelial precursor cells from human fetal neural tissue at a stage at which neurogenesis occurs. Methods are described for culturing of the cells in a medium containing fibroblast growth factor and chick embryo extract, as are methods for immunodepleting from the cultured human fetal neural cells any cells expressing A2B5, NG2 and eNCAM such that an isolated population of human neuroepithelial precursor cells remains. | | Abstract: | A method for isolating human neuroepithelial precursor cells from human fetal tissue by culturing the human fetal cells in fibroblast growth factor and chick embryo extract and immunodepleting from the cultured human fetal cells any cells expressing A2B5, NG2 and eNCAM is provided. In addition, methods for transplanting these cells into an animal are provided. Animals models transplanted with these human neuroepithelial precursor cells and methods for monitoring survival, proliferation, differentiation and migration of the cells in the animal model via detection of human specific markers are also provided. | | US Patent Website: | Click Here for Full Text of Patent | | Title Number: | US6852532 | | Application Number: | US2001000813429 | | Date Filed: | 21/03/2001 | | Date Published: | 08/02/2005 | | Assignee: | University of Utah Research Foundation, Salt Lake City, UT, USA |
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