Methods comprising apoptosis inhibitors for the generation of transgenic pigs

Stem Cell Clinic
Patient Application
FAQ
Contact
Locations
News
Videos
Research
Patents
3D Culture/Scaffold
Administration
Differentiation
Expansion
Extraction/Preservation
Mobilization
Type
USE
 


Stem Cell Related Patent Number US6852532

Title:Method of isolating human neuroepithelial precursor cells from human fetal tissue
Inventors:Mayer-Proschel, Margot; Pittsford, NY, USA
Rao, Mahendra S.; Salt Lake City, UT, USA
Tresco, Patrick A.; Sandy, UT, USA
Messina, Darin J.; Salt Lake City, UT, USA
Summary:This is a novel system for isolating human neuroepithelial precursor cells from human fetal neural tissue at a stage at which neurogenesis occurs. Methods are described for culturing of the cells in a medium containing fibroblast growth factor and chick embryo extract, as are methods for immunodepleting from the cultured human fetal neural cells any cells expressing A2B5, NG2 and eNCAM such that an isolated population of human neuroepithelial precursor cells remains.
Abstract:A method for isolating human neuroepithelial precursor cells from human fetal tissue by culturing the human fetal cells in fibroblast growth factor and chick embryo extract and immunodepleting from the cultured human fetal cells any cells expressing A2B5, NG2 and eNCAM is provided. In addition, methods for transplanting these cells into an animal are provided. Animals models transplanted with these human neuroepithelial precursor cells and methods for monitoring survival, proliferation, differentiation and migration of the cells in the animal model via detection of human specific markers are also provided.
US Patent Website:Click Here for Full Text of Patent
Title Number:US6852532
Application Number:US2001000813429
Date Filed:21/03/2001
Date Published:08/02/2005
Assignee:University of Utah Research Foundation, Salt Lake City, UT, USA


 
Copyright © 2007 The Institute for Cellular Medicine  6/18/2021