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Stem Cell Related Patent Number US6852534

Title:Method to determine an engrafting cell dose of hematopoietic stem cell transplant units
Inventors:Wernet, Peter; Düsseldorf, Germany
Kögler, Gesine; Düsseldorf, Germany
Fischer, Johannes; Neuss, Germany
Summary:This invention describes a method of determining an engrafting cell dose of hematopoietic stem cell transplant units from transplant sources having nucleated cells that have been selected from a group consisting of cord blood, bone marrow, and peripheral blood cells. Further disclosed are the means by which the source of such nucleated cells is subjected to a substantially complete erythrocyte lysis, from which measurement are conducted in a cell counter of a signal corresponding selectively to white blood cells. Also provided are methods of assessing quantitatively the nucleated red blood cell (NRBC) count as part of the total nucleated cell (NC) count, from which the number of white blood cells (WBCs) is determined as transplant-relevant cells. Therapeutic applications are included, such as in the determination of whether a white blood cell count is transplant-relevant by subjecting its source to the erythrocyte lysis described herein.
Abstract:A method to determine an engrafting cell dose of hematopoietic stem cell transplant units from transplant sources having nucleated cells selected from the group consisting of cord blood, bone marrow, peripheral blood comprising the steps of
    • subjecting the source to a substantially complete erythrocyte lysis,
    • measuring in a cell counter a signal corresponding selectively to white blood cells,
    • assessing essentially quantitatively nucleated red blood cell (NRBC) count as part of the total nucleated cell (NC) count,
    • and determining the number of white blood cells (WBCs) as transplant relevant cells.
subjecting the source to a substantially complete erythrocyte lysis, measuring in a cell counter a signal corresponding selectively to white blood cells, assessing essentially quantitatively nucleated red blood cell (NRBC) count as part of the total nucleated cell (NC) count, and determining the number of white blood cells (WBCs) as transplant relevant cells.
US Patent Website:Click Here for Full Text of Patent
Title Number:US6852534
Application Number:US2001000985334
Date Filed:02/11/2001
Date Published:08/02/2005
Assignee:Kourion Therapeutics GmbH, Duesseldorf, Germany


 
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