Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US6863885

Title:Method of decreasing radiation or radio-mimetic chemotherapy for hematopoietic pluripotent cell engraftment
Inventors:Mardiney, III, Michael; Baltimore, MD, USA
Summary:Described herein is a method of engrafting hematopoietic pluripotent stem cells with a reduced radiation dosage following treatment of the donor with a hematopoietic growth factor. The invention relates to a method of engrafting donor mammalian hematopoietic pluripotent cells in a mammalian recipient such that at least one dosage of a hematopoietic growth factor is administered, after which the recipient is subjected to a low dosage of radiation, which is followed by transplantation of the donor hematopoietic pluripotent cells in the recipient. Precise means of engraftment via the decreased amount of radiomimetic compound are disclosed, as are the properties of the hematopoietic growth factor, which is selected from the group consisting of the granulocyte colony stimulating factor (GCSF), the stem cell factor (SCF), the granulocyte macrophage colony stimulating factor (GMCSF), interleukin-1 (IL-1), interleukin-3 (IL-3), interleukin-6 (IL-6), interleukin-8 (IL-8), interleukin-11 (IL-11), interleukin-12 (IL-12), leukemia inhibitory factor (LIF), fibroblast growth factor-?(FGF-?) and FMS-like tyrosine kinase 3-ligand (FLT3-ligand), or a combination thereof. After the recipient receives a non-myeloablative amount of the radiomimetic compound, the transplantation of the hematopoietic pluripotent cells into the recipient thereby yields a successful engrafting of the allogenic donor mammalian hematopoietic pluripotent cells. Also described are therapeutic applications such as in the treatment of heematopoietic disease such as aplasia or thalassemia.
Abstract:The present invention provides a method of engrafting donor mammalian hematopoietic pluripotent cells in a mammalian recipient using a decreased amount of radiation, comprising: (a) administering to the recipient at least one dosage of a hematopoietic growth factor; (b) subjecting the recipient to a low dosage of radiation; and (c) transplanting the donor hematopoietic pluripotent cells in the recipient, thereby engrafting the donor mammalian hematopoietic pluripotent cells in the mammalian recipient using a decreased amount of radiation. The invention also provides a method of engrafting donor mammalian hematopoietic pluripotent cells in a mammalian recipient using a decreased amount of radiomimetic compound, comprising: (a) administering to the recipient at least one dosage of a hematopoietic growth factor; (b) subjecting the recipient to a low dosage of radiomimetic compound; and (c) transplanting the donor hematopoietic pluripotent cells in the recipient, thereby engrafting the donor mammalian hematopoietic pluripotent cells in the mammalian recipient using a decreased amount of radiomimetic compound.
US Patent Website:Click Here for Full Text of Patent
Title Number:US6863885
Application Number:US2002000201212
Date Filed:22/07/2002
Date Published:08/03/2005
Assignee:The USA as represented by the Department of Health and Human Services, Washington, DC, USA


 
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