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Stem Cell Related Patent Number US6887706

Title:Method of in vitro differentiation of transplantable neural precursor cells from primate embryonic stem cells
Inventors:Zhang, Su-Chun; Middleton, WI, USA
Thomson, James A.; Madison, WI, USA
Duncan, Ian David; Madison, WI, USA
Summary:Described herein are methods of differentiating primate embryonic stem cells into neural precursor cells. The invention relates to the propagation of stem cells from a primate embryonic stem cell culture, and methods of culturing the embryoid bodies in a medium containing fibroblast growth factor 2, from which the neural precursor cells will be generated. Methods of isolation are further disclosed.
Abstract:A method of differentiating embryonic stem cells into neural precursor cells is disclosed. In one embodiment the method comprises the steps of (a) obtaining an embryonic stem cell culture, (b) propagating the stem cells, (c) forming embryoid bodies from the stem cells, and (d) culturing the embryoid bodies in a medium containing an effective amount of fibroblast growth factor 2, wherein neural precursor cells will be generated and isolated.
US Patent Website:Click Here for Full Text of Patent
Title Number:US6887706
Application Number:US2001000970382
Date Filed:03/10/2001
Date Published:03/05/2005
Assignee:Wisconsin Alumni Research Foundation, Madison, WI, USA


 
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