Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US6890728

Title:Methods of isolating blood components using a microcentrifuge and uses thereof
Inventors:Dolecek, Victor D.; Englewood, CO, USA
Marx, Jeffery C.; Germantown, TN, USA
McKay, William F.; Memphis, TN, USA
Summary:Described herein are methods of collecting predetermined cellular components from bodily fluid or tissue via the use of centrifugation incorporating a rotatable flexible centrifuge bag and centrifuge. The invention relates to an apparatus and methods by which predetermined cellular components are separated from a bodily fluid or tissue by centrifugation, such that the cellular components may include stem cells, buffy coat, bone marrow cells, erythrocytes, leukocytes, thrombocytes, serum, fluorescently labeled cells, and magnetically labeled cells, from whole blood, bone marrow, buffy coat, fat tissue, muscle tissue, and nerve tissue. Methods of labeling and centrifugation are disclosed, by which the components are separated.
Abstract:Methods and apparatuses for the separation a predetermined cellular component from a bodily fluid or tissue by centrifugation are provided. More specifically, this invention provides a method for the separation and isolation of one or more components, such as stem cells, buffy coat, bone marrow cells, erythrocytes, leukocytes, thrombocytes, serum, fluorescently labeled cells, and magnetically labeled cells, from whole blood, bone marrow, buffy coat, fat tissue, muscle tissue, and nerve tissue by centrifugation, wherein the components are isolated while the centrifuge is rotating.
US Patent Website:Click Here for Full Text of Patent
Title Number:US6890728
Application Number:US2002000116729
Date Filed:04/04/2002
Date Published:10/05/2005
Assignee:Medtronic, Inc., Minneapolis, MN, USA


 
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