Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US6897061

Title:Transdifferentiation of glial cells
Inventors:Salin-Nordstrom, Tuija Helina; Santa Monica, CA, USA
Summary:Herein is described a method of in vitro transdifferentiation of mammalian cells from glial cells to neurons, oligodendrocytes and astrocytes. The invention relates to methods for culturing the cells to form a group of cells and exposing the cells to a growth factor. The in vitro techniques provide for the dedifferentiation of fetal or adult mammalian glial cells into multipotent cells, which may further be differentiated into particular types of nervous system cells such as those mentioned above. Methods are disclosed by which a small sample of astrocytes is used to establish an in vitro culture of cells that is expanded and processed to yield multipotent cells, which in turn may be used directly or be further differentiated to yield neurons and/or oligodendrocytes and/or astrocytes. Also described are methods of transplantation of the generated cells into patients.
Abstract:A process for generating multipotent cells from glial cells using in vitro techniques to dedifferentiate fetal or adult mammalian glial cells into multipotent cells. The multipotent cells may further be differentiated into particular types of nervous system cells, including neurons, astrocytes, and oligodendrocytes. A small sample of astrocytes is used to establish an in vitro culture of cells that is expanded and processed to yield multipotent cells that may be used directly or be differentiated to yield neurons and/or oligodendrocytes and/or astrocytes. The invention includes implanting the generated cells into patients. The invention includes a step of exposing the cells to a growth factor.
US Patent Website:Click Here for Full Text of Patent
Title Number:US6897061
Application Number:US2000000644498
Date Filed:23/08/2000
Date Published:24/05/2005
Assignee:Spinal Cord Society, Fergus Falls, MN, USA


 
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