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| Title: | Conjugated ligands for the stimulation of blood cell proliferation by effecting dimerization of the receptor for stem cell factor |
| Inventors: | Hendrickson, Wayne A.; New York, NY, USA Jiang, Xuliang; Braintree, MA, USA Langley, Keith E.; Newbury Park, CA, USA Syed, Rashid; Thousand Oaks, CA, USA Hsu, Yueh-Rong Ann; Westlake, CA, USA |
| Summary: | Described herein are methods and materials for the preparation of a compound that binds to the stem cell factor (SCF)-binding site of the SCF receptor, by identification of the SCF receptor-binding site on SCF fragments based on its three-dimensional structure. The invention relates to a computer based method for preparing a stem cell factor (SCF) analog, such that a computer expression of the three-dimensional structure of an SCF molecule is provided from its crystal structure, from which the SCF molecule is then prepared after the computer expression has been selected, the SCF molecule having an alteration of at least one selected site. Optional methods are provided by which the SCF molecule may be tested for a desired characteristic. Further described are means by which SCF analogs and SCF ligand analogs may be prepared according to this method. Therapeutic applications are included, such as in the design of compounds capable of binding to the SCF receptor site, and which are produced according to this method. |
| Abstract: | This invention provides a computer based method for preparing a stem cell factor (SCF) analog comprising the steps of: (a) providing computer expression of the three dimensional structure of an SCF molecule using its crystal structure; (b) selecting from the computer expression of step (a) at least one site on the SCF molecule for alteration; (c) preparing a SCF molecule having an alteration at said at least one selected site; and (d) optionally, testing the SCF molecule for a desired characteristic. This invention also provides SCF analogs and SCF ligand analogs prepared according to the above-described method. Compositions comprising SCF analogs or SCF ligand analogs prepared according to the above-described method effective to treat a subject and a pharmaceutically acceptable carrier are provided, as are methods of treating a subject comprising administration of pharmaceutical compositions comprising the prepared SCF analogs and SCF ligand analogs prepared by the described methods. This invention also provides methods for designing compounds capable of binding to the SCF receptor site and compounds designed by the above-described methods. |
| US Patent Website: | Click Here for Full Text of Patent |
| Title Number: | US6904369 |
| Application Number: | US2000000609027 |
| Date Filed: | 29/06/2000 |
| Date Published: | 07/06/2005 |
| Assignee: | The Trustees of Columbia University in the City of New York, New York, NY, USA Amgen Inc., Thousand Oaks, CA, USA |