Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US6908763

Title:Mammalian common lymphoid progenitor cell
Inventors:Akashi, Koichi; Chestnut Hill, MA, USA
Kondo, Motonari; Redwood City, CA, USA
Weissman, Irving L.; Redwood City, CA, USA
Summary:This invention describes enriched mammalian common lymphoid progenitor cells with progenitor cell activity for lymphoid lineages but lacking the potential to differentiate into myeloid and erythroid lineages. Also provided are methods of isolation and culture of such a common lymphoid progenitor cell (CLP) population, such that reagents are employed which specifically recognize the CDw127 (IL-7 receptor alpha) and CD117 (c-kit) protein in conjunction with other markers expressed on lineage committed cells. Also included are murine cells characterized as expressing low levels of sca-1 (Ly-6E and Ly-6A). The CLPs are described as being predominantly cycling, blast cells which give rise to B cells, T cells and natural killer cells.
Abstract:A substantially enriched mammalian hematopoietic cell subpopulation is provided, which is characterized by progenitor cell activity for lymphoid lineages, but lacking the potential to differentiate into myeloid and erythroid lineages. Methods are provided for the isolation and culture of this common lymphoid progenitor cell (CLP). The cell enrichment methods employ reagents that specifically recognize CDw127 (IL-7 receptor α); CD117 (c-kit) protein, in conjunction with other markers expressed on lineage committed cells. The murine cells are also characterized as expressing low levels of sca-1 (Ly-6E and Ly-6A). The CLPs are predominantly cycling, blast cells. These cells give rise to B cells, T cells and natural killer cells, as evidenced by their growth and differentiation in vitro and in vivo.
US Patent Website:Click Here for Full Text of Patent
Title Number:US6908763
Application Number:US1997000918537
Date Filed:22/08/1997
Date Published:21/06/2005
Assignee:The Board of Trustees of the Leland Stanford Junior University, Palo Alto, CA, USA


 
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