Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US6909030

Title:PTTG knockout rodent as a model to study mechanisms for various physiological phenomena, including diabetes
Inventors:Melmed, Shlomo; Los Angeles, CA, USA
Wang, Zhiyong; Los Angeles, CA, USA
Summary:Described herein is a novel null mutant (knockout) rodent, comprising in its germ cells an artificially induced pituitary tumor transforming gene (PTTG) null mutation. The invention relates to methods by which such a rodent can be generated by way of homologous recombination in an embryonic stem cell or germ cell. Further described are applications in the research of mammalian physiology at the cellular, tissue, and/or organismal level with respect to various phenotypes, such as hyperglycemia, hypoinsulinaemia, hypoleptinemia, diabetes, chromosomal aneuploidy, premature centromere division, chromosomal damage, aberrant mitotic cellular division, thrombocytopenia, thymic hyperplasia, splenic hypoplasia, testicular hypoplasia, and female subfertility. The invention further relates to an animal model for diabetes, and also disclosed is a somatic or germ cell, as well as a cell line, obtained from the null mutant rodent. Applications in the study of diabetes are included.
Abstract:Disclosed is a null mutant (or knockout) rodent comprising in its germ cells an artificially induced PTTG null mutation. In some embodiments, the null mutant rodent can be generated by way of homologous recombination in an embryonic stem cell or germ cell. The inventive null mutant rodent can be used to study mammalian physiology at the cellular, tissue, and/or organismal level with respect to various phenotypes, including hyperglycemia, hypoinsulinaemia, hypoleptinemia, diabetes, chromosomal aneuploidy, premature centromere division, chromosomal damage, aberrant mitotic cellular division, thrombocytopenia, thymic hyperplasia, splenic hypoplasia, testicular hypoplasia, and female subfertility. Also disclosed is an animal model for diabetes. Also disclosed is a somatic or germ cell obtained from the null mutant rodent. Also disclosed is a cell line derived from a cell obtained from the null mutant rodent.
US Patent Website:Click Here for Full Text of Patent
Title Number:US6909030
Application Number:US2001000978146
Date Filed:15/10/2001
Date Published:21/06/2005
Assignee:Cedars-Sinai Medical Center, Los Angeles, CA, USA


 
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