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Stem Cell Related Patent Number US6924146
| Title: | Method of constructing vectors for homologous recombination directed mutagenesis | | Inventors: | Wattler, Sigrid; The Woodlands, TX, USA
Nehls, Michael; The Woodlands, TX, USA | | Summary: | Described herein is a novel insertional mutagenesis system comprised of a linear lambda vector and a positive selection cassette vector. The invention relates to a novel vector system and method for the simplified construction of recombinant vectors for directed mutagenesis, specifically in the modification of the eukaryotic genome of embryonic stem cells, at precise and predefined loci by means of homologous recombination. Further disclosed are properties of the vector comprising an E. Coli origin of replication, an antibiotic resistance gene, and a selectable marker suitable for use in yeast. Therapeutic applications are included such as in gene therapy and in the modification of eukaryotic genomes, especially in the creation of transgenic animals. | | Abstract: | The present invention provides a novel vector system and thereby a novel method for the simplified construction of recombinant vectors for directed mutagenesis. Said vector system is used to modify the eukaryotic genome, particularly of embryonic stem cells, at precise and predefined loci by the means of homologous recombination. Furthermore, said system finds its usage in the generation of new strategies for gene therapy and in the generation of genetically modified higher eukaryotic organisms. | | US Patent Website: | Click Here for Full Text of Patent | | Title Number: | US6924146 | | Application Number: | US1998000171642 | | Date Filed: | 20/02/1998 | | Date Published: | 02/08/2005 | | Assignee: | None |
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