Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US6929948

Title:Lineage specific cells and progenitor cells
Inventors:Smith, Austin G.; Edinburgh, United Kingdom
Li, Meng; Edinburgh, United Kingdom
Summary:Described herein are methods of generating a purified or enriched culture. The invention relates to methods by which cells of a selected lineage are generated from which a selectable marker, which is differentially expressed when compared with its expression in other cells, is introduced into a multipotent cell which is then cultured to induce differentiation into a cell of the selected lineage, or into a mixture of cells including cells of the selected lineage. Methods are further provided by which the multipotent cell is alternately cultured to induce preferential survival of cells of the selected lineage, such that those cells expressing the selectable marker are then selected. Therapeutic applications are included, such as in the transplantation of cells and genetic selection techniques that are employed to eliminate non-neural cells.
Abstract:A method for generating a culture that is purified or enriched in respect of cells of a selected lineage is described in which selectable marker, which is differentially expressed in cells of the selected lineage compared with its expression in other cells, is introduced into a multipotential cell and the multipotential cell is cultured to induce differentiation of the multipotential cell into a cell of the selected lineage, or into a mixture of cells including cells of the selected lineage, or is cultured to induce preferential survival of cells of the selected lineage. Those cells that express the selectable marker are then selected for. Progenitors of selected lineage are also described as is the use of the method in assay techniques.
US Patent Website:Click Here for Full Text of Patent
Title Number:US6929948
Application Number:US2000000686880
Date Filed:12/10/2000
Date Published:16/08/2005
Assignee:The University Court of the University of Edinburgh United Kingdom


 
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