Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US6942995

Title:Methods of creating constructs useful for introducing sequences into embryonic stem cells
Inventors:Klein, Robert D.; Palo Alto, CA, USA
Brennan, Thomas J.; South San Francisco, CA, USA
Summary:This invention describes a method of creating a nucleotide construct which contains a positive selection marker flanked by restriction sites and sequences that can be treated to produce single stranded regions and which is useful for introducing targeted mutations into embryonic stem cells. The invention provides novel nucleotide constructs and the methods by which DNA constructs are made, such that sequences are introduced into the function of a gene in a cell, particularly in an embryonic stem cell, for purposes of disrupting that function. Therapeutic applications are included.
Abstract:This invention provides novel nucleotide constructs. Also provided are methods for making DNA constructs useful for introducing sequences into and disrupting the function of a gene in a cell, particularly an embryonic stem cell.
US Patent Website:Click Here for Full Text of Patent
Title Number:US6942995
Application Number:US2002000087523
Date Filed:28/02/2002
Date Published:13/09/2005
Assignee:Deltagen, Inc., San Carlos, CA, USA


 
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