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Stem Cell Related Patent Number US6955802

Title:Homeodomain protein code specifying progenitor cell identify and neuronal fate in the ventral neural tube
Inventors:Jessell, Thomas M.; Bronx, NY, USA
Briscoe, James; London, United Kingdom
Ericson, Johan; Hasselby, Sweden
Summary:Described herein is a novel genetically engineered cell for the treatment of motor neuron injury, comprising a neural stem cell and a retroviral expression system which expresses homeodomain transcription factor Nkx6.1 but does not express Irx3/Nkx2.2. This invention relates to genetically engineered cells comprising a neural stem cell and a retroviral expression system in the neural stem cell, which is capable of expressing homeodomain transcription factor Nkx6.1 protein but does not express homeodomain transcription factor Irx3 protein or homeodomain transcription factor Nkx2.2 protein. Further described are methods of generating such genetically engineered motor neurons, V2 neurons, and V3 neurons, as well as methods of treating subjects having a motor neuron injury or a motor neuron disease via the implantation into injured or diseased neural tissue of the subject any of the provided genetically engineered cells, such that the neural tissue retroviral expression systems are capable of expressing the appropriate homeodomain proteins. Also provided are methods of transfecting neural stem cells with a retroviral vector, which is capable of expressing the required homeodomain transcription factor proteins. Further disclosed is a method of determining whether a chemical compound affects the generation of a motor neuron from a neural stem cell.
Abstract:Provided are genetically engineered cells comprising a neural stem cell and retroviral expression system in the neural stem cell, which is capable of expressing homeodomain transcription factor Nkx6.1 protein but does not express homeodomain transcription factor Irx3 protein or homeodomain transcription factor Nkx2.2 protein; which is capable of expressing homeodomain transcription factor Nkx6.1 protein and homeodomain transcription factor Irx3 protein; and which is capable of expressing homeodomain transcription factor Nkx2.2 protein or homeodomain transcription factor Nkx2.9 protein. Also provided are methods of generating such genetically engineered motor neurons, V2 neurons, and V3 neurons. Also provided are methods of treating subjects having a motor neuron injury or a motor neuron disease comprising implanting in injured/diseased neural tissue of the subject any of the provided genetically engineered cells, administering to such neural tissue retroviral expression systems which are capable of expressing the appropriate homeodomain protein(s), or transfecting neural stem cells with a retroviral vector, which is capable of expressing the required homeodomain transcription factor protein(s). Provided is a method of determining whether a chemical compound affects the generation of a motor neuron from a neural stem cell.
US Patent Website:Click Here for Full Text of Patent
Title Number:US6955802
Application Number:US2000000569259
Date Filed:11/05/2000
Date Published:18/10/2005
Assignee:The Trustees of Columbia University in the City of New York, New York, NY, USA


 
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