Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US6960446

Title:Method for isolating cells expressing flk-2
Inventors:Lemischka, Ihor R.; Princeton, NJ, USA
Summary:This invention describes a novel method for the isolation of protein tyrosine kinase as it is expressed in primitive hematopoietic cells (HC) and not expressed in mature HC. Also disclosed are ligands for the protein and for the stimulated proliferation of primitive hematopoietic stem cells. Central to the invention are isolated mammalian nucleic acid molecules encoding receptor protein tyrosine kinases expressed in primitive hematopoietic cells and not expressed in mature hematopoietic cells. Further included are the receptors encoded by such nucleic acid molecules which encode receptor protein tyrosine kinases having the sequences comprising murine flk-2, human flk-2, and murine flk-1. Also provided are methods of stimulating the proliferation and/or differentiation of primitive mammalian hematopoietic stem cells via contacting the stem cells with a ligand that binds to the receptor protein tyrosine kinase.
Abstract:Isolated mammalian nucleic acid molecules encoding receptor protein tyrosine kinases expressed in primitive hematopoietic cells and not expressed in mature hematopoietic cells are provided. Also included are the receptors encoded by such nucleic acid molecules; the nucleic acid molecules encoding receptor protein tyrosine kinases having the sequences shown in FIG. 1a (murine flk-2), FIG. 1b (human flk-2) and FIG. 2 (murine flk-1); the receptor protein tyrosine kinases having the amino acid sequences shown in FIG. 1a, FIG. 1b and FIG. 2; ligands for the receptors; nucleic acid sequences that encode the ligands; and methods of stimulating the proliferation and/or differentiation of primitive mammalian hematopoietic stem cells comprising contacting the stem cells with a ligand that binds to a receptor protein tyrosine kinase expressed in primitive mammalian hematopoietic cells and not expressed in mature hematopoietic cells.
US Patent Website:Click Here for Full Text of Patent
Title Number:US6960446
Application Number:US2001000919408
Date Filed:31/07/2001
Date Published:01/11/2005
Assignee:The Trustees of Princeton University, Princeton, NJ, USA


 
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