Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US6972195

Title:Composition and method for culturing potentially regenerative cells and functional tissue-organs in vitro
Inventors:Xu, Rongxiang; Arcadia, CA, USA
Summary:This is a method for culturing cells in vitro, such that tissue cells are isolated from a predetermined site of the body of a mammal, for which a tissue culture medium is prepared through a novel combination of fatty acids and a sterol compound, from which the isolated tissue cells are cultured.
Abstract:Compositions and methods are provided for culturing in vitro potentially regenerative cells (PRCs) from which functional tissue-organs are regenerated. In one aspect of the invention, a tissue culture medium is provided which comprises at least 50% of water and a sterol compound that is dissolved in a fatty acid-containing oil at a concentration at least 0.1% by weight based on the weight of the oil and added to the water. The culture medium can be used to culture PRCs that are isolated from the body of a mammal to generate functional tissue-organs in vitro with substantially the same physiological structure and function as the corresponding ones existing in vivo and in situ. The cultured PRCs, tissues, and tissue-organs can serve as valuable models for scientific investigation in life sciences, nutraceutical discovery, drug screening, pharmacokinetic studies, medical devices and tissue/organ transplantation.
US Patent Website:Click Here for Full Text of Patent
Title Number:US6972195
Application Number:US2002000335143
Date Filed:31/12/2002
Date Published:06/12/2005
Assignee:Rongxiang Xu, Arcadia, CA, USA


 
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