Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US7029913

Title:Primate embryonic stem cells
Inventors:Thomson, James A.; Madison, WI, USA
Summary:This invention describes a purified preparation of primate embryonic stem cells, characterized by the cell surface markers SSEA-1 (-), SSEA-4 (+), TRA-1-60 (+), TRA-1-81 (+), and alkaline phosphatase (+). Disclosed herein are methods by which such cells are human embryonic stem cells with normal karyotypes, which continue to proliferate in an undifferentiated state after continuous culture for eleven months. The embryonic stem cell lines also retain the ability to form trophoblast and to differentiate into all tissues derived from all three embryonic germ layers, namely, the endoderm, mesoderm and ectoderm. Further described is a method for isolating a primate embryonic stem cell line. Therapeutic applications are described in the generation of transgenic non-human primates for models of specific human genetic diseases or for tissue transplantation
Abstract:A purified preparation of primate embryonic stem cells is disclosed. This preparation is characterized by the following cell surface markers: SSEA-1 (−); SSEA-4 (+); TRA-1-60 (+); TRA-1-81 (+); and alkaline phosphatase (+). In a particularly advantageous embodiment, the cells of the preparation are human embryonic stem cells, have normal karyotypes, and continue to proliferate in an undifferentiated state after continuous culture for eleven months. The embryonic stem cell lines also retain the ability, throughout the culture, to form trophoblast and to differentiate into all tissues derived from all three embryonic germ layers (endoderm, mesoderm and ectoderm). A method for isolating a primate embryonic stem cell line is also disclosed.
US Patent Website:Click Here for Full Text of Patent
Title Number:US7029913
Application Number:US2001000982637
Date Filed:18/10/2001
Date Published:18/04/2006
Assignee:Wisconsin Alumni Research Foundation, Madison, WI, USA


 
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