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| Title: | Hematopoietic stem cell proliferating agents |
| Inventors: | Saito, Yoshimasa; Kawanishi, Japan Ueda, Yoshiko; Osaka, Japan Tamura, Kouichi; Kobe, Japan Takata, Yoko; Osaka, Japan Yamada, Hisashi; Sanda, Japan Yamashita, Tatsuo; Kobe, Japan Kobayashi, Masakazu; Takarazuka, Japan |
| Summary: | Described herein is a hematopoietic stem cell proliferation agent containing IGF-I, and its uses in the culturing of hematopoietic stem cells. The invention relates to a method of culturing hematopoietic stem cells in a medium containing IGF-I and at least one protein selected from the group consisting of SCF and M-CSF. Further disclosed are methods of proliferating the hematopoietic stem cells in a mammal and administering IGF-I with at least one protein selected from the group consisting of M-CSF and G-CSF to the mammal, in an amount sufficient to stimulate hematopoietic stem cell proliferation. |
| Abstract: | This invention relates to a hematopoietic stem cell proliferating agent comprising IGF-I, a hematopoietic stem cell proliferating agent comprising IGF-I and at least one protein selected from among SCF, M-CSF, and G-CSF, and a method of growing hematopoietic stem cells which comprises culturing hematopoietic stem cells in a medium containing IGF-I and at least one protein selected from the group consisting of SCF and M-CSF. The hematopoietic stem cell proliferating agent of the invention causes hematopoietic stem cells to proliferate in the undifferentiated state whether in vivo or in vitro and can, therefore, be used for amelioration of the cytopenia induced by radiotherapy or chemotherapy using anticancer drugs, prevention of infectious diseases associated with lymphopenia, or in vitro culture for multiplication of hematopoietic stem cells and extrasomatic culture of recombinant stem cells in gene therapy. [From equivalent US20030068820A1] This invention relates to a hematopoietic stem cell proliferating agent comprising IGF-I, a hematopoietic stem cell proliferating agent comprising IGF-I and at least one protein selected from among SCF, M-CSF, and G-CSF, and a method of growing hematopoietic stem cells which comprises culturing hematopoietic stem cells in a medium containing IGF-I and at least one protein selected from the group consisting of SCF and M-CSF. The hematopoietic stem cell proliferating agent of the invention causes hematopoietic stem cells to proliferate in the undifferentiated state whether in vivo or in vitro and can, therefore, be used for amelioration of the cytopenia induced by radiotherapy or chemotherapy using anticancer drugs, prevention of infectious diseases associated with lymphopenia, or in vitro culture for multiplication of hematopoietic stem cells and extrasomatic culture of recombinant stem cells in gene therapy. |
| US Patent Website: | Click Here for Full Text of Patent |
| Title Number: | US7037892 |
| Application Number: | US2002000198355 |
| Date Filed: | 19/07/2002 |
| Date Published: | 02/05/2006 |
| Assignee: | Astellas Pharma Inc., Tokyo, Japan |