Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US7045678

Title:Transgenic mouse for screening therapeutic agents for brain tumors
Inventors:Chiu, Ing-Ming; Dublin, OH, USA
Summary:Described herein is a novel transgenic non-human mammal containing a transgene with a promoter and a nuclear factor binding region of the RR2 cis-acting element of a fibroblast growth factor FGF-1B promoter which is operably linked to DNA encoding the simian virus 40 (SV40) large T antigen. Clinical applications in the screening of drugs are included.
Abstract:A transgenic, non-human mammal useful for assessing the effect of candidate chemotherapeutic drugs on the growth of brain tumors in vivo is provided. Incorporated into the genome of the transgenic mammal, which preferably is a rodent, is a transgene which comprises a promoter comprising the nuclear factor binding region of the RR2 cis acting element of a fibroblast growth factor 1B (FGF1B) promoter. Operably linked to the promoter is reporter gene comprising a sequence which encodes the SV40 large T antigen. A transgenic, non-human mammal useful for identifying and isolating FGF1 producing brain cells. Incorporated into the genome of these transgenic animals is a transgene which comprises a promoter comprising the nuclear factor binding region of the RR2 cis acting element of an fibroblast growth factor 1B (FGF1B) promoter. Operably linked to the promoter is reporter gene comprising a sequence which encodes a protein or polypeptide other than an SV40 large T antigen. A method of obtaining neural stem cells from a sample of cells obtained from an animal is also provided. Such method comprises introducing the FGF1B-detector transgene into a sample of cells that have been obtained from the animal, and assaying for expression of the detectable marker in the cells, wherein cells that express the marker are neural stem cells. The cells which express the detectable marker can then be isolated from the population to provide a sub-population of neural stem cells.
US Patent Website:Click Here for Full Text of Patent
Title Number:US7045678
Application Number:US2004000829491
Date Filed:22/04/2004
Date Published:16/05/2006
Assignee:None


 
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