Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US7067316

Title:Methods and devices for the long-term culture of hematopoietic progenitor cells
Inventors:Pykett, Mark J.; Boxford, MA, USA
Rosenzweig, Michael; Boston, MA, USA
Kaplan, Richard B.; Beverly Hills, CA, USA
Summary:Described herein is a process for the long-term in vitro culturing of hematopoietic progenitor cells. The invention relates to culturing in the absence of exogenously added hematopoietic growth factors, thereby improving upon methods for the introduction of foreign genetic material into cells of hematopoietic origin. Also disclosed is the apparatus for performing such methods, which incorporates a three-dimensional porous biomaterial matrix which supports transduction and enhances hematopoietic progenitor cell survival, thereby inducing an expansion of the progenitor cell numbers and functionality, while maintaining progenitor cell pluripotency. Applications are provided in promoting the growth, survival and differentiation of stem cells.
Abstract:The invention pertains to methods and devices for the long term, in vitroculture of hematopoietic progenitor cells in the absence of exogenously added hematopoietic growth factors, improved methods for the introduction of foreign genetic material into cells of hematopoietic origin, and to apparatus for performing these methods. The hematopoietic progenitor cells are cultured on a three-dimensional porous biomaterial. The three-dimensional porous biomaterial enhances hematopoietic progenitor cell survival and leads to an expansion of progenitor cell numbers and/or functionality, while maintaining progenitor cell pluripotency in the absence of exogenous growth factors. In addition, the three-dimensional porous biomaterial supports high level transduction on cells cultured upon such environment.
US Patent Website:Click Here for Full Text of Patent
Title Number:US7067316
Application Number:US2003000705720
Date Filed:10/11/2003
Date Published:27/06/2006
Assignee:Cytomatrix, LLC, Woburn, MA, USA


 
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